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Staphylococcus aureus is the leading cause of skin infections in the U.S., and its rapid evolution and resistance to antibiotics create a barrier to effective treatment. In this study, we engineered a composite membrane with bacterial cellulose and carbon nanotubes (BC-CNT) as an electroactive dressing to rapidly eradicate vancomycin-intermediate S. aureus. Nonpathogenic Komagataeibacter sucrofermentans produced the BC membrane at an air-liquid interface. Then, carboxyl-functionalized multi-walled CNTs were integrated into decellularized BC to create stable and electrically conductive BC-CNT dressings. The electric potential and ionic flux across BC-CNT were modeled and standardized via chronoamperometry for experimental validation. We found that treatment with electroactive BC-CNT increases S. aureus sensitivity to vancomycin and prevents macro-scale biofilm formation. The bactericidal efficacy of the composite membrane is consistent with electrochemical stress caused by voltage mediated with BC-CNT. After a single hour of combinatorial electrical and drug treatment, biofilm-forming capacity was inhibited by nearly 92 %. These results advance applications of electrochemistry in medicine and create a new direction to overcome S. aureus infections on skin and soft tissues. 

While reactor wall preconditioning was previously shown to influence the growth of carbon nanotubes (CNTs) by chemical vapor deposition (CVD), it was previously only limited to studying the accumulating carbon deposits over the history of a large number of growth runs. However, the effect of leaving the reactor walls for an extended period of time between growth runs was not previously systematically studied. Here, we combine experimental measurements with a mathematical model to investigate the effect of thermochemical history of reactor walls on growth yield of vertically aligned CNT forests. Importantly, we demonstrate unexpectedly high CNT yield, exceeding one-order-of-magnitude taller forests, by increasing the interim period between runs (IPBR). We explain the results based on previously unexplored process sensitivity to trace amounts of oxygen-containing species in the reactor. In particular, uncontrolled amounts of water vapor desorbing from reactor walls during growth are modelled in this work. Our modeling results show the effect of IPBR on the outgassing dynamics revealing the underlying mechanism of generating growth promoting molecules during growth. By installing a new humidity sensor in our multizone rapid thermal CVD reactor, we are able to uniquely correlate the amount of moisture within the reactor to real-time measurements of growth kinetics, as well as ex situ characterization of CNT alignment and atomic defects. Our findings enable a scientifically grounded approach toward both boosting growth yield and improving its consistency by reducing run-to-run variations. Accordingly, engineered growth recipes can be envisioned to leverage this effect for improving manufacturing process scalability and robustness. 

Abstract Origami-based fabrication strategies open the door for developing new manufacturing processes capable of producing complex three-dimensional (3D) geometries from two-dimensional (2D) sheets. Nevertheless, for these methods to translate into scalable manufacturing processes, rapid techniques for creating controlled folds are needed. In this work, we propose a new approach for controlled self-folding of shape memory polymer sheets based on direct laser rastering. We demonstrate that rapidly moving a CO2 laser over pre-strained polystyrene sheets results in creating controlled folds along the laser path. Laser interaction with the polymer induces localized heating above the glass transition temperature with a temperature gradient across the thickness of the thin sheets. This gradient of temperature results in a gradient of shrinkage owing to the viscoelastic relaxation of the polymer, favoring folding toward the hotter side (toward the laser source). We study the influence of laser power, rastering speed, fluence, and the number of passes on the fold angle. Moreover, we investigate process parameters that produce the highest quality folds with minimal undesired deformations. Our results show that we can create clean folds up to and exceeding 90 deg, which highlights the potential of our approach for creating lightweight 3D geometries with smooth surface finishes that are challenging to create using 3D printing methods. Hence, laser-induced self-folding of polymers is an inherently mass-customizable approach to manufacturing, especially when combined with cutting for integration of origami and kirigami. 

Abstract The purpose of this paper is to characterize the dynamics and direction of self-folding of pre-strained polystyrene (PSPS) and non-pre-strained styrene (NPS), which results from local shrinkage using a new process of directed self-folding of polymer sheets based on a resistively heated ribbon that is in contact with the sheets. A temperature gradient across the thickness of this shape memory polymer (SMP) sheet induces folding along the line of contact with the heating ribbon. Varying the electric current changes the degree of folding and the extent of local material flow. This method can be used to create practical three-dimensional (3D) structures. Sheets of PSPS and NPS were cut to 10 × 20 mm samples, and their folding angles were plotted with respect to time, as obtained from in situ videography. In addition, the use of polyimide tape (Kapton) was investigated for controlling the direction of self-folding. Results show that folding happens on the opposite side of the sample with respect to the tape, regardless of which side the heating ribbon is on, or whether gravity is opposing the folding direction. The results are quantitatively explained using a viscoelastic finite element model capable of describing bidirectional folds arising from the interplay between viscoelastic relaxation and strain mismatch between polystyrene and polyimide. Given the tunability of fold times and the extent of local material flow, resistive-heat-assisted folding is a promising approach for manufacturing complex 3D lightweight structures by origami engineering.